Federica Albanese

4 Questions 4 Answers 0 Followers

Questions related from Federica Albanese

How many images I need to acquire for TEM experiments in vitro?

Dear all, I am imaging HEK cells using TEM for the first time. I have currently seeded the cells into a 6-well plate and I am using a single well as n=1. So cells are a monolayer and the facility...

22 February 2023 3,866 4 View

How do you measure Mitophagy in HEK cells?

Hi, I am starting studying Mitophagy, so I was looking into some publication to see how to measure mitophagy reliably. Like which kind of markers do you usually use? is colocalization of LC3B and...

19 May 2021 1,054 5 View

Why do protein and mRNA levels not always correlate with each other?

Hello, I have performed WB analysis and qPCR analysis to analyze the protein level and the rispective gene relative expression of mTOR, GBA1, LC3 genes in brain of mutant mice. I observed a...

02 December 2020 4,723 2 View

Why does LC3-II not increase after CQ treatment in vivo?

I have administered CQ treatment (50 mg/kg) in twelve-months-old C57BL/6J mice to assess autophagic flux. I validated this protocol on 3-months-old mice first, obtaining an accumulation of LC3II...

06 April 2020 5,217 3 View