What is the difference between single-cell RNA-Seq and typical RNA-Seq in terms of data analysis procedure?

Hello, I am working on some single-cell RNA-Seq samples and wondering what the difference between a typical RNA-Seq analysis and single-cell RNA-Seq analysis is? Can we use standard Subread and...

31 December 2019 2,585 3 View

Why do unicellular organisms and cultured cells use aerobic glycolysis?

I am studying about relations between warburg effect and cancer evolution and find this question. Why do unicellular organisms and cultured cells use aerobic glycolysis?

31 December 2016 6,963 0 View

Are scientists able to remove immortality from cancer cells?

Are scientists able to take immortality from cancer cells? what is your comment about this article: Scientists from the Spanish National Cancer Research Centre (CNIO) have discovered a new...

11 December 2015 3,385 4 View

May we hope to use immortality of cancer cells for create immortal normal cells?

11 December 2015 1,764 1 View

What is the hot topics in the field of cancer genetics?

As a molecular genetics student I would like to ask this great community to advice what is the hot topics in the field of cancer genetics, cancer stem cells, biomarkers and generally the best...

11 December 2015 5,508 0 View

Can we modify mortal cells to immortal cells or control aging by avoiding a telomere crisis?

11 December 2015 4,097 12 View

How we can use Transmission Electron Microscopy (TEM) for studying cancer and biomarkers of cancer?

what is the usage of TEM for studying cancer and Biomarkers? for understanding new aspect of cancer how we can use TEM? there anyone that work with electron microscopy for studying cancer which...

11 December 2014 9,068 0 View

What is the last method for staining of DNA and DNA detection in ultra structure samples for Electron Microscopy (EM)?

We are already studying DNA and chromatin with different methods (For example using Osmium Ammine for DNA staining) but I want ask if someone also work in this field with the same methods or there...

11 December 2014 4,824 9 View

What are the functions of free calcium in the nucleus and nuclei?

10 November 2014 2,436 7 View

We are looking for Calcium concentration in the HeLa cells nucleus with TEM, Do you know any methods for staining free Ca2+ in nucleus?

09 October 2014 7,580 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View