what is the usage of TEM for studying cancer and Biomarkers? for understanding new aspect of cancer how we can use TEM?
there anyone that work with electron microscopy for studying cancer which can give me more detail?
Hello, I am working on some single-cell RNA-Seq samples and wondering what the difference between a typical RNA-Seq analysis and single-cell RNA-Seq analysis is? Can we use standard Subread and...
31 December 2019 2,752 3 View
Regarding to the Functions of the Warburg Effect and tumour microenvironment also enhances disruptions of tissue architecture and immune cell evasion. Why do oncogenes induce the Warburg Effect...
31 December 2016 5,776 0 View
I am studying about relations between warburg effect and cancer evolution and find this question. Why do unicellular organisms and cultured cells use aerobic glycolysis?
31 December 2016 7,052 0 View
Are scientists able to take immortality from cancer cells? what is your comment about this article: Scientists from the Spanish National Cancer Research Centre (CNIO) have discovered a new...
11 December 2015 3,463 4 View
I am wondering to know if it is possible to arrive a point of genetics in the future which we will use immortality feature of cancer cells to modify normal cells to immortal cells?
11 December 2015 1,851 1 View
As a molecular genetics student I would like to ask this great community to advice what is the hot topics in the field of cancer genetics, cancer stem cells, biomarkers and generally the best...
11 December 2015 5,589 0 View
The few cancer cells that are immortal activate telomerase reverse transcriptase (TERT), a part of the telomerase enzyme that rebuilds telomeres so they avoid a telomere crisis. Normally, TERT is...
11 December 2015 4,210 12 View
We are already studying DNA and chromatin with different methods (For example using Osmium Ammine for DNA staining) but I want ask if someone also work in this field with the same methods or there...
11 December 2014 4,898 9 View
What are the functions of free calcium in the nucleus and nuclei as a regulation factor for transcription? How does it work? Has somebody done projects related to Ca in the nucleus?
10 November 2014 2,510 7 View
We want to know localization of Calcium in the nucleus or nucleolus or chromatin of HeLa cells and also looking for staining but after staining in the transmission electron microscopy we see lot...
09 October 2014 7,676 7 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View