What is the difference between single-cell RNA-Seq and typical RNA-Seq in terms of data analysis procedure?

Hello, I am working on some single-cell RNA-Seq samples and wondering what the difference between a typical RNA-Seq analysis and single-cell RNA-Seq analysis is? Can we use standard Subread and...

31 December 2019 2,585 3 View

Why do oncogenes induce the Warburg Effect cell intrinsically?

31 December 2016 5,701 0 View

Why do unicellular organisms and cultured cells use aerobic glycolysis?

I am studying about relations between warburg effect and cancer evolution and find this question. Why do unicellular organisms and cultured cells use aerobic glycolysis?

31 December 2016 6,963 0 View

Are scientists able to remove immortality from cancer cells?

Are scientists able to take immortality from cancer cells? what is your comment about this article: Scientists from the Spanish National Cancer Research Centre (CNIO) have discovered a new...

11 December 2015 3,385 4 View

May we hope to use immortality of cancer cells for create immortal normal cells?

11 December 2015 1,764 1 View

What is the hot topics in the field of cancer genetics?

As a molecular genetics student I would like to ask this great community to advice what is the hot topics in the field of cancer genetics, cancer stem cells, biomarkers and generally the best...

11 December 2015 5,508 0 View

Can we modify mortal cells to immortal cells or control aging by avoiding a telomere crisis?

11 December 2015 4,097 12 View

What is the last method for staining of DNA and DNA detection in ultra structure samples for Electron Microscopy (EM)?

We are already studying DNA and chromatin with different methods (For example using Osmium Ammine for DNA staining) but I want ask if someone also work in this field with the same methods or there...

11 December 2014 4,824 9 View

What are the functions of free calcium in the nucleus and nuclei?

10 November 2014 2,436 7 View

We are looking for Calcium concentration in the HeLa cells nucleus with TEM, Do you know any methods for staining free Ca2+ in nucleus?

09 October 2014 7,580 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View