What is the difference between single-cell RNA-Seq and typical RNA-Seq in terms of data analysis procedure?

Hello, I am working on some single-cell RNA-Seq samples and wondering what the difference between a typical RNA-Seq analysis and single-cell RNA-Seq analysis is? Can we use standard Subread and...

31 December 2019 2,585 3 View

Why do oncogenes induce the Warburg Effect cell intrinsically?

31 December 2016 5,701 0 View

Why do unicellular organisms and cultured cells use aerobic glycolysis?

I am studying about relations between warburg effect and cancer evolution and find this question. Why do unicellular organisms and cultured cells use aerobic glycolysis?

31 December 2016 6,963 0 View

Are scientists able to remove immortality from cancer cells?

Are scientists able to take immortality from cancer cells? what is your comment about this article: Scientists from the Spanish National Cancer Research Centre (CNIO) have discovered a new...

11 December 2015 3,385 4 View

May we hope to use immortality of cancer cells for create immortal normal cells?

11 December 2015 1,764 1 View

Can we modify mortal cells to immortal cells or control aging by avoiding a telomere crisis?

11 December 2015 4,097 12 View

How we can use Transmission Electron Microscopy (TEM) for studying cancer and biomarkers of cancer?

what is the usage of TEM for studying cancer and Biomarkers? for understanding new aspect of cancer how we can use TEM? there anyone that work with electron microscopy for studying cancer which...

11 December 2014 9,068 0 View

What is the last method for staining of DNA and DNA detection in ultra structure samples for Electron Microscopy (EM)?

We are already studying DNA and chromatin with different methods (For example using Osmium Ammine for DNA staining) but I want ask if someone also work in this field with the same methods or there...

11 December 2014 4,824 9 View

What are the functions of free calcium in the nucleus and nuclei?

10 November 2014 2,436 7 View

We are looking for Calcium concentration in the HeLa cells nucleus with TEM, Do you know any methods for staining free Ca2+ in nucleus?

09 October 2014 7,580 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View