I'm using wax moth larvae for some soil baiting experiment, and I'm not sure why some larvae seem to "forget" to moult one last time, and die in failing to pupate, stuck in hardening exoskeleton. Apart from a paper reporting that cooling larvae lead some to fail to pupate, I can't find any information on the subject. As I'm looking at mortality rate in soils and more larvae seem to fail to pupate in the sterile control, I'd like more information.

Thank you very much,

Celine

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