Istkhar Rao

9 Questions 34 Answers 0 Followers

Questions related from Istkhar Rao

Which classification of insects hemocytes should be followed?

I am working with insect hemocytes. for identification, classification of Gupta (1994) and Johns (1959) was applied in previous studies with ambiguities. Now which classification is good. Can...

06 September 2016 587 13 View

What are the standards of entomopathogenic nematodes Identification?

Can anybody tell about the standards set for the identification of entomopathogenic nematodes. Thanks in advance.

26 May 2016 6,095 5 View

How to extract endotoxins from bacterial culture?

I am working with symbiont bacteria of steinernematid and heterorhrabditid nematodes and want to extract their endotoxins. Kindly provide the procedure for extraction as well as for their...

13 April 2016 2,388 7 View

How to make 10% phenylthiourea (PTU) solution for phenoloxidase activity test?

I am working on hemolymph of lepidopteran insect and want to calculate the PO activity in the hemolymph. For control group, I need to add 10% PTU solution, but it is not soluble in water/ PBS. How...

16 March 2016 9,370 4 View

What will be the correct decision when results of molecular Identification of EPNs revealed from COI gene oppose the results of ITS and D2D3 regions?

I am working on the identification of entomopathogenic nematodes (EPNs). During the molecular identification of the species, I found 100% similarity of the studied EPN isolates with Steinernema...

06 November 2015 5,125 2 View

Does anyone suggest to how to find the nucleotide starting and ending site of miscellaneous rDNA within a amplified sequence for ITS region?

I have some amplified sequences of ribosomal DNA which contain 28S rRNA, ITS1, 5.8s rRNA, ITS2 and 28S rRNA, but I dont know to how to find the starting and ending position of each region...

02 April 2015 3,227 4 View

I'm encountering coagulation of hemocyte with anti-coagulant buffer, does anyone have a solution?

I am interested to calculate the total and differential hemocytes of lepidopteran insects. I am using a protocol prepared by myself in the lab, where the use of anti-coagulant buffer (98mM NaoH,...

31 January 2015 2,456 4 View

From where can I get the of larvae of Helicoverpa armigera for culture purposes in Laboratory?

I am trying to study the immune response of Insects against nematode parasite for which I need to culture the insect Helicoverpa armigera the target pest of my study. I am looking for someone who...

09 January 2015 504 3 View

Can anyone recommend a protocol for extraction methods of total lipid, protein, and carbohydrate from microscopic nematode?

I am working on entomopathogenic nematodes. What are the best method to extract the total lipid carbohydrate and proteins from these tiny nematode? Is there any kit available in the market for...

15 August 2014 8,639 3 View