What is the best way to isolate proteins which are located in the endosomes and the ER membrane?

For one of my projects, I need to perform WBs on cell lysates. The protein of interest (LRAT) is located in the endosomes and the membrane of the ER. What is the best protocol to isolate proteins...

25 November 2020 969 3 View

How to increase the signal of the protein standard when performing western blot?

Hi, I'm writing my master's thesis on the characterization of human proteins which I express and purify from E. coli. I'm performing western blot to verify my protein, but the protein standard...

06 October 2020 1,107 5 View

Tips on interpreting cell morphology and counting mouse primary aortic smooth muscle cells?

Hello, I recently started culturing mouse primary aortic smooth muscle cells (purchased from Cell Biologics) with the media supplied by the same company (5% FBS and a whole lot of other stuff)....

19 March 2020 9,781 3 View

What is the best method to prepare copper nanoparticles ?

I want a simple method for preparation of copper nanoparticles. The methods mentioned in publications have something missing or something they didn't mention. So if anyone has a practical...

07 September 2019 7,313 4 View

Can someone help with human aortic smooth muscle cells (HASMC)?

Hello, I just started working with human aortic smooth muscle cells (HASMC) for the first time and experienced contamination problems. I purchased Lonza's human smooth muscle cell systems with...

17 February 2019 809 2 View

Isolating high molecular weight DNA from a single feather?

I'm attempting to extract DNA from a single retrix from a small sparrow. I have upwards of 200 samples but only one feather per individual. The feathers have no visible blood spots on them. I've...

14 August 2018 5,190 2 View

Why an actin band does not appear on gel after PCR for BMM diffrerentiated into osteoclasts?

Hello everyone, I am currently trying to set up at a new lab in order to begin experiments on osteoclasts. Before starting anything, I wanted to make sure that BMMs have indeed differentiated...

17 May 2018 3,308 3 View

How to augmented virtual contents on on-device uploaded images?

Dear all, I need some help regarding image recognition and/or augmented reality. I don't want to use to scan images with camera to augment it with virtual content. Instead, I would like to upload...

17 January 2018 671 1 View

Is there any significant difference between Silver and Au as electrodes for polymer thin films dielectric constant measurements?

12 June 2017 3,702 3 View

How Adiponectine blood level could be increased?

Adiponectin (APN) is associated with insulin sensitivity. one way to increase the insulin sensitivity is to increase APN in blood, therefore which food could be used to do this directly or indirectly?

04 February 2017 9,011 3 View

Can downdraft gasifier for RDF be scaled up?

Dear researchers, In literature, so far I am seeing that downdraft is usually used for small-scale purposes (10kW-10MW). One of the reasons behind is "they do not allow for uniform distribution...

08 February 2021 5,403 1 View

Petrel- well coordinates issue?

Hi, After loading 2D seismic lines in Petrel, I added three exploratory wells ( which supposed to be within the same 2D area) but these wells appears off the interesting area when I visualized...

25 October 2020 8,272 4 View

What are these bioclasts?

I'm having trouble identifying the round bioclasts indicated by white arrows. Could they be transverse sections of the elements indicated by black arrows? If so, could they be some kind of spicule?

22 July 2020 8,595 13 View

What are the data needed for an EOR study to run simulation on Eclipse simulator and Petrel ?

I am an Msc Student and chose to study what is the best EOR strategies for Ekofisk oil field by using eclipse simulator and petrel software but im confused about exactly what data and information...

10 April 2020 6,271 3 View

Where can I find researchs on facies models about primary volcaniclastics rocks?

I am looking for new research on facies models, similar to those published quite some time ago in the book "Volcanic Successions, Modern and Ancient" and in the book "Pyroclastic Density Currents...

06 July 2019 2,004 3 View

Where can I find researchs on facies models about primary volcaniclastics rocks?

I am looking for new research on facies models, similar to those published quite some time ago in the book "Volcanic Successions, Modern and Ancient" and in the book "Pyroclastic Density Currents...

03 July 2019 2,483 1 View

Salt dome modelling in Petrel?

Dear All, Does anyone know how to treat salt domes in the gridding process of Petrel? I have my horizons as regular surfaces and my salt intrusion is triangle mesh but I fail to incorporate the...

16 April 2019 1,898 4 View

Petrel- 2D seismic data loading?

Hi, While loading 2D seismic lines in Petrel, I get warning on "Duplicate traces". If I continue, the displayed section shows like in blocks. I have seen the segy files in Seisviewer and Vista,...

08 February 2019 9,650 4 View

What can I do to improve my virus production efficiency?

Hello all, During the last few months I tried to make a lenti virus with my target gene. (I used this protocol by Addgene : https://www.addgene.org/protocols/lentivirus-production/). For the...

31 December 2018 1,486 5 View

How can I build a 3D velocity model (on a previously constructed 3D structural framework) using only checkshots in Petrel software?

I'm trying to build a 3D velocity model (on a previously constructed 3D structural framework) in Petrel Software using only checkshots. Can anyone help me, please?

17 November 2018 7,138 36 View