Why are Clarevit or Scopus magazines slow to respond?

When submitting a research to one of Scopus or Clarevit or Thomson Reuter magazines, the journal is delayed in informing the researcher that it has received the research, while any other journal...

11 December 2018 1,605 3 View

How can I show an ID to ORCID when writing a research journal?

Can you explain how this is done....

08 September 2018 9,381 4 View

What is the best program to display tabular data values in a graphic format?

I have used one of the computer programs to calculate the results of my work have appeared in a number of columns of values and I want to display in a curved, what is the appropriate program to...

07 August 2018 1,987 1 View

What are the magazines that can be published within the specialization of mathematics and have monthly publications?

Can you give me information about journals that have monthly publications and do not prolong the period in the evaluation of research and acceptance of publication?

07 August 2018 7,104 2 View

What are the periodic conferences that will be held in the month of August or September?

Can you help me find a conference with a close date and can I send a summary and complete research in these months? Note that the required conference in the discipline of mathematics

06 July 2018 8,550 21 View

What software is the best to analyze non-standard organisms?

Hello, What software is the best to analyze non-standard organisms?

10 November 2017 477 1 View

Western blotting technology?

07 August 2017 4,533 2 View

What is the importance of Q1 as a measurement of journals reputation?

Hello all, What is the importance of Q1 as a measurement of journals reputation? Thank you

05 June 2017 1,136 1 View

Lab animals question?

04 May 2017 1,899 1 View

The heterozygous deletion of Atf4?

03 April 2017 2,255 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View