The heterozygous deletion of Atf4?

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B-spline interpolation for robot trajectory planning?

In B-spline interpolation, how control points and knot vector influences on trajectory optimization for robot path planning?

23 November 2023 1,221 1 View

Is anybody conducting an experimental study on the effects of the product and the process approaches to teaching EFL listening?

The purpose being to determine which of these two approaches has a more positive effect on the development of students'EFL listening.

18 October 2022 7,202 2 View

What could be the source of sudden but persistent low frequency fluctuations on EEG signals?

Hello, Recently, during the EEG signal recording, a low-frequency fluctuation with a very high amplitude, as shown in the attached video, is added to the EEG signals. It suddenly appears during...

27 May 2022 7,123 9 View

Is there any evidence that using ball milling in intervals, rather than one long run, improves the mixing process?

I am trying to synthesize nanoparticles by mixing the precursors by ball milling. I would like to know if mixing them for one hour straight is less or more efficient than mixing them in intervals...

10 November 2020 8,723 9 View

Why are Clarevit or Scopus magazines slow to respond?

When submitting a research to one of Scopus or Clarevit or Thomson Reuter magazines, the journal is delayed in informing the researcher that it has received the research, while any other journal...

11 December 2018 1,785 3 View

How can I show an ID to ORCID when writing a research journal?

Can you explain how this is done....

08 September 2018 9,568 4 View

What is the best program to display tabular data values in a graphic format?

I have used one of the computer programs to calculate the results of my work have appeared in a number of columns of values and I want to display in a curved, what is the appropriate program to...

07 August 2018 2,136 1 View

What are the magazines that can be published within the specialization of mathematics and have monthly publications?

Can you give me information about journals that have monthly publications and do not prolong the period in the evaluation of research and acceptance of publication?

07 August 2018 7,223 2 View

What are the periodic conferences that will be held in the month of August or September?

Can you help me find a conference with a close date and can I send a summary and complete research in these months? Note that the required conference in the discipline of mathematics

06 July 2018 8,690 21 View

RNA-Seq question??

Hello, Does Galaxy assign reads to different isoforms of a gene?

23 November 2017 2,280 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View