Is there any evidence that using ball milling in intervals, rather than one long run, improves the mixing process?

I am trying to synthesize nanoparticles by mixing the precursors by ball milling. I would like to know if mixing them for one hour straight is less or more efficient than mixing them in intervals...

10 November 2020 8,527 9 View

Why are Clarevit or Scopus magazines slow to respond?

When submitting a research to one of Scopus or Clarevit or Thomson Reuter magazines, the journal is delayed in informing the researcher that it has received the research, while any other journal...

11 December 2018 1,605 3 View

How can I show an ID to ORCID when writing a research journal?

Can you explain how this is done....

08 September 2018 9,381 4 View

What is the best program to display tabular data values in a graphic format?

I have used one of the computer programs to calculate the results of my work have appeared in a number of columns of values and I want to display in a curved, what is the appropriate program to...

07 August 2018 1,987 1 View

What are the magazines that can be published within the specialization of mathematics and have monthly publications?

Can you give me information about journals that have monthly publications and do not prolong the period in the evaluation of research and acceptance of publication?

07 August 2018 7,104 2 View

What are the periodic conferences that will be held in the month of August or September?

Can you help me find a conference with a close date and can I send a summary and complete research in these months? Note that the required conference in the discipline of mathematics

06 July 2018 8,550 21 View

What software is the best to analyze non-standard organisms?

Hello, What software is the best to analyze non-standard organisms?

10 November 2017 477 1 View

Western blotting technology?

07 August 2017 4,533 2 View

What is the importance of Q1 as a measurement of journals reputation?

Hello all, What is the importance of Q1 as a measurement of journals reputation? Thank you

05 June 2017 1,136 1 View

Lab animals question?

04 May 2017 1,899 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View