Hi,
I am using AO direct count method. By surfing i studied that cells with RNA/ssDNA fluoresce red in colour and dsDNA fluoresce green. There are arguments that dna/rna content doesn't play significant role. Please suggest.
Dear Saranya,
first of all I would like to point you to sources contained already in RG:
https://www.researchgate.net/search.Search.html?type=question&query=Acridine+orange
You'll find (most probably) not only valuable information, but also answer to your question....
For the shortest general info: cf. https://en.wikipedia.org/wiki/Acridine_orange
In addition to the answers already provided by Prof. Muss, may be the attached articles can be of help for you.
Best regards
Robert
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