Mouse brain endothelial cells bEnd.3 have changed their morphology and do not perform ability to form a fully integral monolayer. It happened after 1,5 years exposure to freezing in nitrogen vapors.
Hello, I noticed a problem with my HPLC analysis and wanted to ask for help. During my analysis I've noticed repetitive problems with the baseline: it is dropping and then (usually) going back...
20 May 2024 6,501 3 View
Hello I am looking for information about osmophilie microorganisms and specifically their enzymes. If there are any industrially important enzymes from microorganisms living in environment where...
19 May 2024 136 1 View
I am preparing an experiment in which I would like to sample roots of sunflowers grown in pots (substrate is soil) in later stages of the plant development. Can anyone tell me, what is the best...
29 April 2024 8,775 1 View
Hi, Does my TaqMan probe recognize the gene only if the sequence has 100% coverage with the probe or will I also get signal if there is a SNP or even a few bp difference? And if a few bp are ok...
09 April 2024 6,856 3 View
I have tested enzyme activity (b-galactosidase) with cation ions in different buffer and the results differ dramatically. For example, while I used MES buffer calcium acts as an activator but when...
11 February 2024 3,135 2 View
Hello, Does anyone have any kind of experience with ordering cell lines from Accegen? I'm wondering if they are trustworthy and about the quality of provided cell lines?
26 November 2023 3,757 1 View
I think that mentoring programmes support in meeting publishers' expectations.
05 October 2023 2,584 1 View
I need to get information about the number of chromosomes or DNA content. I have seedlings from in vitro that were frozen at -20 degrees after liquidation of the culture. They are seedlings from...
28 September 2023 6,373 2 View
I need to detect phosphorylated IGF1R in cell lysate and brain lysate with Western Blot. Are there any life hacks for detection of such proteins?
05 September 2023 7,310 0 View
https://webmagazine.unitn.it/en/evento/sociologia/115480/esa-rn12-environment-and-society-summer-school
10 March 2023 3,806 3 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,934 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
05 August 2024 8,056 1 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View