Are preprint articles in "bioRxiv" (operated by Cold Spring Harbor Laboratory) citable?

The site (https://www.biorxiv.org/about-biorxiv) mentions that these articles are not peer-reviewed, edited, or typeset before being posted online. However, there are these articles in bioRxiv...

01 February 2019 8,687 1 View

How to interpret minimum free energy (MFE) values for miRNA:mRNA duplex?

What does a higher negative ddG and a positive ddG indicate with regards to binding of miRNA to mRNA? What can be used as a reasonable threshold of MFE to signify actual disruption or enhancement...

08 September 2018 9,031 0 View

What is the half life of microRNAs?

10 November 2016 5,082 1 View

Why is uracil-N-glycosylase used in qPCR?

07 August 2015 5,612 4 View

Which are the databases with experimentally validated miRNAs?

Also are there any sources to know the tissue-specific expression of miRNAs in mouse and human?

31 December 2014 9,913 3 View

Can anyone help with Cotton or Polyester swabs for DNA isolation from human cheek cells?

06 July 2014 5,643 3 View

How does one use PHASE 2.1 for calculating recombination rate, in Win OS?

I need to calculate the recombination rate for the biallelic SNPs in a gene of interest. However, I would like to run the PHASE programe on a Windows OS. How can we make the .inp file? Do we need...

06 July 2014 2,757 0 View

Suggestions for using Sox-2 antibody [(Y-17): SC-17320, SC] for WB ?

Hi All, I'm trying to do a western blot analysis for the detection of Sox-2 in stem cell lines, but i have failed many times over. I kindly need some help and suggestions. I'm using Sox-2...

09 October 2011 8,194 4 View

DNase treatment of LCM RNA

Hi All, After great difficulty I was finally able to isolate RNA from Laser Capture micro-dissected samples using Trizol method, (trials with Micro kits were unsuccessful). my Total RNA yield...

06 July 2011 9,065 8 View

Comprehensive database for epigenetic genes in human and mouse

Dear All, I am looking for a Comprehensive database for all the known epigenetic genes in human and mouse. Though I did come across some which were specific for certain types of cancers, I...

05 June 2011 9,561 0 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View