Can anyone suggest suitable references that discuss or empirically prove this phenomenon, with regards to the role of microRNAs in the human genome?
The site (https://www.biorxiv.org/about-biorxiv) mentions that these articles are not peer-reviewed, edited, or typeset before being posted online. However, there are these articles in bioRxiv...
01 February 2019 8,805 1 View
What does a higher negative ddG and a positive ddG indicate with regards to binding of miRNA to mRNA? What can be used as a reasonable threshold of MFE to signify actual disruption or enhancement...
08 September 2018 9,128 0 View
Does imperfect base-pairing delay microRNA degradation in animals, as compared to plants that have a perfect base-pairing of miRNAs with target mRNAs?
10 November 2016 5,180 1 View
Likewise, Uracil-N-glycosylase cannot be used in One-Step RT-PCR. But it can be used in Two-Step RT-PCR if dUTP is not used in the RT step. Do some RT mixes use dUTP for reverse transcribing RNA?...
07 August 2015 5,786 4 View
Also are there any sources to know the tissue-specific expression of miRNAs in mouse and human?
31 December 2014 10,018 3 View
Hi, could anyone suggest if cotton or polyester swabs would enable greater recovery of buccal cells and the subsequent higher quantity of DNA isolated?
06 July 2014 5,740 3 View
I need to calculate the recombination rate for the biallelic SNPs in a gene of interest. However, I would like to run the PHASE programe on a Windows OS. How can we make the .inp file? Do we need...
06 July 2014 2,862 0 View
Hi All, I'm trying to do a western blot analysis for the detection of Sox-2 in stem cell lines, but i have failed many times over. I kindly need some help and suggestions. I'm using Sox-2...
09 October 2011 8,286 4 View
Hi All, After great difficulty I was finally able to isolate RNA from Laser Capture micro-dissected samples using Trizol method, (trials with Micro kits were unsuccessful). my Total RNA yield...
06 July 2011 9,164 8 View
Dear All, I am looking for a Comprehensive database for all the known epigenetic genes in human and mouse. Though I did come across some which were specific for certain types of cancers, I...
05 June 2011 9,660 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View