Note: elution been done via Qiagen gel extraction kit.
i have to study the awareness and adoption level of cloud computing in a district of India. i also want to use association among demographic variables like gender, age, education, income etc and...
02 August 2024 2,420 3 View
Steel processing industry releases waste water, which contains 20% of Iron with 2 pH. can you suggest any low-cost technique to separate or recover anyone, either acid or Fe from the waste water, pls
24 April 2024 5,636 6 View
Our samples had been analysed by Mothur + Silva 119 reference DB around 2015. When analysing the same samples in 2023 with CLC genomics workbench and SILVA 119 DB, the results are strikingly...
21 March 2024 991 1 View
For a better teaching in the class.
19 February 2024 274 1 View
I have cloned my genes in a CMV vector and transfected them in HEK293 cells. After harvesting the cells, the expression level at the transcriptional level is increased compared to the control...
23 December 2023 2,507 10 View
During storage of Ethanol (USP Grage) after filtration through 5-micron filter membrane. Particulate matter observed during storage. For that, we need to investigate the reason. Is any science...
26 November 2023 2,540 3 View
In one of our pregnane ring structure related product (Drospirenone), we are facing one impurity (i.e. 6-Methyl Drospirenone impurity) problem. The observed level of impurity is about 1% on USP...
25 November 2023 5,102 16 View
how can i form the schematic diagram of g quadruplex as shown in figure?
24 November 2023 1,851 1 View
how to plot binding energy residue with respect to residue number in mmpbsa? i have done mmpbsa for dna-ligand complex. i want to calculate binding energy for every residue. Thanks in advance.
27 October 2023 3,605 1 View
Suppose, I have indicator x1 which is part of a reflective construct A, can I also use x1 as an indicator for another reflective construct B.
26 October 2023 4,318 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
