Greetings! I think you need to dilute your samples before running the qPCR and check the reaction conditions (reaction composition, annealing temperature). The melting curves clearly show the melting of genomic DNA at ~95 C.

Hi

I agree with the comments above. The rRNA expression level is very high and you need to dilute to get rid of low Ct Values. Also the samples must be first optimized on a regular PCR for amplification of desired amplicons before actually going for qPCR assay.

you may have post pcr contaminaion of one of your reagents. What does your no template control sample look like? Does it amplufy?

Thank all of you so much for the helpful opinions. Yes, we always get amplification signals for NTCs. We were told that contamination was likely from DNA polymerase.