Yabing H Nollet

2 Questions 3 Answers 0 Followers

Questions related from Yabing H Nollet

Why do we have low PCR efficiency?

We are trying to quantify ammonia-oxidizing bacteria (AOB) and total bacterial (TB) in wastewater. We use gBlcok gene fragments to construct standard curves. For some reason, we rarely get the...

15 June 2023 9,853 2 View

Why did we get very low Cq values but no melting peaks for wastewater samples?

We are trying to quantify 16S rRNA gene of total bacteria in wastewater samples by using 341f/534r primers in qPCR assay. We got okay standard curves despite the efficiency was unsatisfactory...

23 December 2022 5,627 4 View