We measure the SOD2 in blood of healthy people before and after a supplementation with these vitamins and minerals (vit E, B-carotene, vit C, Zn, Cu and Se, without Mn).
After 4 weeks the levels of Manganese SOD increased significantly, and we don't know why this happen.
Thank you!
Interesting findings. How are you measuring SOD activity and do you see evidence for increases in MnSOD protein in Western Blots or native gels staining for SOD activity? Many SOD assays are prone to minor interferences, specifically with some of these agents you mention. Confirm increases with an orthogonal assay before you go too much further.
Too much antioxidant does not necessary always good to the body. Our own study in endothelial cells show that total SOD2 increase does not mean SOD2 activity will increase. As a matter of fact, only the SOD2 enters into mitochondrial really finctional.
If you overload with metals this may not produce anti-oxidant activity. Metals not incorporated into proteins may generate superoxide. Are you measuring superoxide?
Dear Wayne, Ru-Jeng and John. Thank you for your answers.
Wayne: We are not measuring the SOD activity, we only measure the SOD levels by ELISA.
John: We do not measure the superoxide levels, but we measure the 8-Isoprostane levels, and this decreases after the supplementation.
We have severals economic problems in Venezuela, so our budget for this research is very low. Also, we have a several exchange currency control, so we can't buy another kit's for determination. We do what we can.
Thank you so much for your answer
- These vitamins and minerals in excess concentrations turn out to be pro-oxidants.
- The preparations might have metal contamination.
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