I've been working with the BT474 cell line recently. However, following microscopic observation, I was set on subculturing it the next day. The next day, I discovered that the majority of the cells had detached from the surface and had formed clumps that were floating on the growth media, albeit a small number of cells had remained attached to the surface. The culture flask showed no signs of contamination.
Please make some recommendations.
Thank you so much in advance.