I've been working with the BT474 cell line recently. However, following microscopic observation, I was set on subculturing it the next day. The next day, I discovered that the majority of the cells had detached from the surface and had formed clumps that were floating on the growth media, albeit a small number of cells had remained attached to the surface. The culture flask showed no signs of contamination.

Please make some recommendations.

Thank you so much in advance.

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