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Questions related from Md. Shahidul Islam
I am now subculturing the Raji cell line (ATCC), however, I am having trouble since after splitting the cell line for subculturing, I saw that the cells were in the dead phase even though the cell...
03 February 2023 6,170 2 View
I am now working with the HUVEC cell line. I made my complete media this way like F12K nutrient mix with 10% FBS, 0.1 mg/mL heparin sodium salt, and 0.03 mg/mL endothelial cell growth supplement...
17 May 2022 2,601 0 View
I've been working with the BT474 cell line recently. However, following microscopic observation, I was set on subculturing it the next day. The next day, I discovered that the majority of the...
02 March 2022 2,187 1 View
Currently, I am using CCK-8 to check cell viability on my desired assay plates. Can I, however, reuse the cells after using this reagent for additional experiments with this cell line? Give some...
11 October 2021 2,599 4 View
Currently, I'm working with the RAW 264.7 cell line. We used to use Trypsin-EDTA to remove cells from the surface of other cell lines, however, ATCC recommended using a cell scraper instead of...
25 September 2021 3,810 2 View
I'm currently working with a BT474 cell line, but my cell line was confluent, and I forgot to wash it through PBS, thus I trypsinized it. As a result, even though I trypsinized my cells, they...
01 September 2021 629 4 View
I'm now dealing with the NFS-60 cell line, which is a suspension cell line, however, it continues to grow and shows that it is attached to the culture flask surface despite the fact that I changed...
17 August 2021 3,919 3 View
