What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Do you need to acidify your samples prior to desalting with C18?

I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...

18 October 2020 6,639 3 View

Sampling rate in matlab?

Can I know what is the sampling rate of ifft() command which is a build in function in matlab?

01 June 2020 7,937 12 View

Finding Hidden Defect in ceramic sample in matlab?

I am dealing with ' Optimal signal processing of frequency-stepped CW radar data' from the website of Optimal Signal Processing of Frequency-Stepped CW Radar Data I want to find the hidden...

27 May 2020 1,125 12 View

Any experts in NVIVO 12 Plus for Qualitative data analysis?

I have interview contents that need NVIVO to compile and provide tools for data analysis and presentation. Need experts to share software application experience, pros and cons, advantages and...

01 March 2019 8,723 4 View

Which journals in applied linguistics have short turnaround times? How long was your longest wait...?

Which journals in applied linguistics have short turnaround times? How long was your longest wait from the first draft submission till the first substantial response from the editor / reviewers?

16 December 2018 5,102 12 View

Which lysis buffer should I use for blotting proteins found in the ER and Golgi?

I am looking to see if various proteins of interest are mis-glycosylated, and trapped in the ER or Golgi apparatus. Initially, my experiments have been focussed on performing western blots to look...

29 October 2014 8,893 3 View

NHS reactive group on CyDye and iTRAQ reagents?

Both iTRAQ tags and DiGE CyDye have an NHS reactive group that reacts with primary amines. iTRAQ tags react with the epsilon amino group on lysine and the N terminal group of proteins where as...

01 September 2013 6,613 4 View

My triple quadrupole got corroded. Is there anyway to get it back? Or would it affect my signal a lot?

I saw some brass color and rainbow pattern on my stainless steel quadrupole when I cleaned it. I previously done some analysis of lipids and pesticide with acidic solvent (1% acetic acid in...

20 January 2013 4,278 6 View

The race of world-class university ranking is raises question of worthiness to put in huge investment?

How to be more efficient and cost effective when come to transformation and upgrading university to stay competitive in education market? World ranking is a MUST or just as a marketing tool?

01 January 1970 6,237 8 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View