I am planning to test the activity of a proteinase using an old casein-agar plate assay. According to a protocol I followed, it requires sterilizing the media with casein in it. The first time I did, the media turned black. No aggregate formation, just clear black media(which might have burnt). Can I autoclave the casein+agar?
Also, I am planning to use casein enzymatic hydrolysate instead of casein. Will that make a difference in the activity assay?
P.G. Wiles
Casein is insoluble in water, but soluble in alkaline solution where it forms caseinate. What pH are you using? What form of casein are you using? Acid/lactic casein, or rennet casein? Have you examined what happens if you heat treat the components separately?
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