Haimantika Dey

4 Questions 2 Answers 0 Followers

Questions related from Haimantika Dey

Can one perform an EMSA prior to CLIP-seq?

I wanted to confirm the binding of a lncRNA to a known protein. The protein is mostly disordered except for the part that may be involved in the binding (a coiled-coil region at the end). I was...

26 February 2024 869 1 View

How to solve a structure of a protein that is mostly unstructured?

The majority part of a protein is unstructured (as per the Alphafold model) and just the part that has been seen to bind to a certain component is structured so far. When the protein binds to this...

07 October 2023 3,980 2 View

Why did my casein+agar media turn black after autoclaving?(no aggregrate formation, clear black media)?

I am planning to test the activity of a proteinase using an old casein-agar plate assay. According to a protocol I followed, it requires sterilizing the media with casein in it. The first time I...

06 March 2023 1,522 1 View

Should I dilute my stock protein? If that, then by how much?

My stock protein concentration is 2.13mg/ml. I have to have a final concentration of 0.83 nanomoles and my final volume for the protein would be 50 microlitres. The molecular weight of the protein...

01 March 2023 5,459 3 View