15 October 2015 1 8K Report

What is the best method to get equilibrium interlayer distance of graphite?

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How to store fish tissue sample for proximate analysis and antioxidant enzyme analysis?

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Acute toxicity test is not showing proper results. Want to know what could be the factors?

When we started the experiment at first at 1ppm all the fishes died within 12 hours. repeated the experiments with lower concentrations starting from 0.05 ppm and found a very good response to the...

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How to solve Fuzzy Boundary Value Problem by using Fuzzy Laplace Transform Method?

There are so many papers available to solve initial value problem by using Fuzzy Laplace Transform method. But need solution for the boundary value problem by using the same method.

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Can interpretations based on non-parametric tests be accepted for non-normally distributed sample in the fields of Experimental/Survey Research?

For a non-normally distributed small sample (N

20 November 2023 1,953 4 View

When i going to do Rietveld Refinement in X-pert highscore plus it is showing negetive intensities in scan data points.. Why it is showing?

Actually I want to insert hkl values for my peaks in XRD pattern.. hkl values are not coming automatically in x-pert highscore plus software. So i have to do Rietveld Refinement. But when i am...

12 December 2022 8,784 8 View

What's a nice starting reference on calculation of off-diagonal spin correlations using the directed loop (SSE) algorithm?

How should one normalize the answer correctly, after creating a histogram of the separation between the head and tail of a worm?

07 November 2022 6,542 2 View

How to achieve denaturation and renaturation of protein from pellet fraction of the cell (BL21 DE3)?

I am struggling with a Mycobacterium protein (phthalate esterase), which upon overexpression forms inclusion body (maybe) and it is found in the pellet fraction. I have tried cloning the protein...

27 July 2022 2,716 6 View

Lost protein (phthalate hydrolase) activity which I could achieve once but in later attempts the protein is not showing function? Please suggest??

I have cloned a phthalate hydrolase (~37 kDa) into a pMAL-c2x vector and overexpressed in E.coli BL21(DE3). Induction condition - 28 degrees for 3h with 0.5mM IPTG. This is a Mycolicibacterium...

07 June 2022 6,486 4 View

How to calculate protein content per gram of tissue sample in Fish Tissue?

I have used Lowry method to calculate protein content in fish muscle. Briefly, 5% tissue homogenate is prepared in 0.25 M sucrose. Centrifuged and the supernatant is used for analysis. 0.1 mL of...

31 May 2022 6,205 0 View

How metal is extracted from a leached solution?

I was going though this paper "Extraction of vanadium from high calcium vanadium slag using direct roasting and soda leaching" but finally how could they extract from the leached solution I didn;t...

02 September 2021 4,167 3 View

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