When I want to separate protein by gel electrophoresis i have need to give constant current (I) but in case of DNA separation it is voltage (V). could anyone please explain on that???
Actually, the best is constant Power, so that the product of the current and voltage remains constant so you have constant amount of heating. In real life, all three work though...
I use constant voltage for SDS-PAGE, as recommended by the maker of the precast gels. I use a Novex gel box in which the entire gel is submerged, which presumably helps keep it from overheating.
Interesting question. I use constant voltage for both DNA gel electrophoresis as well as SDS-PAGE, but the values are different in each case. For DNA gel electrophoresis I use a 100V setting while for SDS-PAGE I go for 80V while in stacking gel and 120V when sample is in resolving gel. Like Dirk said, a large part of these values are partly traditional. What you should look out for is that there is no overheating, and that your buffers are not too old.
Interesting question. I use constant voltage for both DNA gel electrophoresis as well as SDS-PAGE, but the values are different in each case. For DNA gel electrophoresis I use a 100V setting while for SDS-PAGE I go for 80V while in stacking gel and 120V when sample is in resolving gel. Like Dirk said, a large part of these values are partly traditional. What you should look out for is that there is no overheating, and that your buffers are not too old.
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