Sanat Myti

5 Questions 7 Answers 0 Followers

Questions related from Sanat Myti

Is Deep well plate suitable for OD600 measurement?

Deep well plates manufactured by NEST Scientific. Materials of construction is USP grade VI Polypropylene. If you have any information please share your knowledge. Thanking you in...

04 April 2019 1,249 3 View

What is the dielectric constant of DMEM with 10% FBS?

I want to perform nanoparticle volume and number distribution measurement where dielectric constant parameter is required. Could anyone please help me by sharing information about this value? Thanks

08 August 2018 824 1 View

Could anyone please help me to give an answer about C-terminal amino acid deletion during protein processing in e. coli?

I am trying to express a protein containing 213 amino acids in e.coli cells. After processing protein will be in periplasmic space. Here we use pelB as a leader sequence. Protein is producing....

04 April 2018 3,296 6 View

Why constant current for protein separation in PAGE and constant voltage for nucleic acid separation????

When I want to separate protein by gel electrophoresis i have need to give constant current (I) but in case of DNA separation it is voltage (V). could anyone please explain on that???

09 September 2016 9,538 5 View

What is the composition of prewash, wash, binding, elution buffer of PCR clean up for DNA recovery ??

Could anyone please give me some information about PCR cleanup buffer composition (Prewash buffer, Wash buffer, Binding buffer, Elution buffer) for DNA recovery using silica-based column?? Thanks...

01 January 1970 2,103 6 View