What is the dielectric constant of DMEM with 10% FBS?

I want to perform nanoparticle volume and number distribution measurement where dielectric constant parameter is required. Could anyone please help me by sharing information about this value? Thanks

07 August 2018 788 1 View

Could anyone please help me to give an answer about C-terminal amino acid deletion during protein processing in e. coli?

I am trying to express a protein containing 213 amino acids in e.coli cells. After processing protein will be in periplasmic space. Here we use pelB as a leader sequence. Protein is producing....

03 April 2018 3,263 6 View

Why constant current for protein separation in PAGE and constant voltage for nucleic acid separation????

When I want to separate protein by gel electrophoresis i have need to give constant current (I) but in case of DNA separation it is voltage (V). could anyone please explain on that???

08 September 2016 9,509 5 View

What is the composition of prewash, wash, binding, elution buffer of PCR clean up for DNA recovery ??

Could anyone please give me some information about PCR cleanup buffer composition (Prewash buffer, Wash buffer, Binding buffer, Elution buffer) for DNA recovery using silica-based column?? Thanks...

01 January 1970 2,066 6 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View