Hi Lim,

Some more information would be helpful.  I found your original gene locus listed in genbank.  Where did you obtain your SNP containing sequence? 

Many tree species have sequence differences between different cultivars, we see that all the time with poplar.

Amy

Hi Amy, 

I get the SNP sequence from a journal's supplementary data. I attached the journal and the SNP detail here. 

Hi Lim,

use sequence data carefully...there are sequencing errors in the submitted as well as published sequences....these may misguide you some times.....make sure the SNPs do exist in the samples that were used in the published paper.

bye