Why buffers are expressed in X rather than molarity?

09 September 2017 0 5K Report

If they are more than one component involved in preparing a buffer then it is expressed in X?

Why are buffer sometimes expressed in X rather than molarity

Badges
Science method

More Xin Pin's questions See All

How to determine cutoff for an IVD assay?

How to determine LoD, LoQ, Linearilty, Cutoff, Expected values for an IVD assay (infectious disease or physiological state monitoring eg. hormones). Has anyone preformed these, can you explain...

01 February 2020 3,145 3 View

Monoclonal antibodies both capture and detection raised in murine?

Hi, I have come across an IVD quantitative assay for C-peptide where both anti analyte biotin-conjugated antibody which binds to Strep coated wells and HRP conjugated anti-analyte antibody was...

31 December 2019 3,778 3 View

Test for pre-existing antibodies against AAV?

If a patient has been exposed to a particular type of AAV already, their immune system might have developed antibodies that could neutralize the effects of that vector. How do I know if a...

02 March 2019 5,180 7 View

How is enzyme unit defined with respective to substrate concentration?

How do i define the exact substrate concentration for 1 U of enzyme. Say my protein concentration after purification is 1mg/ml. If i take 500µl of it (which has 500µg) to convert the...

01 February 2019 462 5 View

CTG assay (CellTiter-Glo) ATP generated?

"The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to...

10 November 2018 4,100 2 View

After passaging the cells for 24 times, what will be the generation number?

After reviving the cells from -80C and seeding the cells ~1 million in T25 flask when the cells reach 80% confluency then if I passage/subculture the cells for eg. spleen (finite) or CHO or...

06 July 2018 597 7 View

Blue White Screening?

Standard cloning strains DH5α, Top10, Novablue or α-Select Gold contain lacZΔM15 deletion mutation? The lacZ gene in the plasmid vector complements the lacZ deletion mutation in the host E. coli...

31 December 2017 3,715 7 View

Surfactant vs Defoamer?

Can surfactant and defoamer be used at the same time?. Surfactant (an amphiphilic) works based on reducing the surface tension of water binding thru hydrophobic interaction and hydrophilic...

31 December 2017 4,027 5 View

Final concetration 10% DMSO?

If I had 28million cells/ml (A) and require 11million cells/ml. I add 392.85 ul from (A) + 607.15 ul of 19.4% (since it is 0.6 times more) DMSO. Now final Conc. of DMSO is 10%? Explain with...

11 December 2017 3,058 1 View

Competetive ELISA result interpretation?

Coated antigen conc. used 50ng/100ul antigen1 (is an antibody) conc. 100ng/100ul + purified Mab conc. 1000ng/100ul antigen1 is an recombinant Mab with human Fc region against peptide conjugated...

10 November 2017 4,725 7 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

What is the best method for removing paraffin from plant samples prepared for microtome?

...

24 July 2024 3,087 3 View