I carried out DNA extraction from skin biopsies of warts using Qiagen's QIAamp DNA Mini Kit. I performed off-column RNase digestion followed by ethanol precipitation.
Similar problem encountered (and suggestions/help) here:
https://www.researchgate.net/post/I_am_getting_two_bands_on_agarose_gel_when_running_genomic_DNA_of_yeast-can_anyone_help
This may be a mechanical shearing of genomic DNA
agree with @ Noha Tharwat Abou El-khier
regards
If the size of lower band is around 8.000bp it may be DNA of human papilloma virus (HPV)
.
I currently can't find any information regarding the relevant restriction enzymes.
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