Bouin’s is generally used for GI, testicular and epididymis fixation because it preserves nuclei and chromosomes better than 10% formaiin. Bouin’s is especially good for observing meiosis.
This soloution is good for fixation of gastrointestinal tract samples because the nuclear staining with it, is better than 10% neutral-buffered formalin (NBF). Althought, It is not a good fixative for ultrastructul studies with electron microscopy.
Bouin's is used for testicular fixation because it preserves nuclei and chromosomes especially well, such as those we would want to observe during meiosis process.
Several studies showed that Bouin’s fluid and Modified Davidson’s fluid (mDF) preserve morphology of the adult animal testis better than formalin. However, mDF has been suggested as a superior substitute for Bouin’s fluid for fixation of adult animal testes. And also, it provided better morphologic detail in the fetal rat testis compared with 10% NBF and Bouin’s fluid in immunostaining protocols.
The general move to using BF as a routine fixative for the testis has certainly improved the general quality of cellular preservation and the resolution of cellular detail that can be achieved, but it presents a number of problems of its own. The presence of picric acid in the fixative results in safety hazards and disposal problems, as well as bright yellow staining of working surfaces and anything that comes in contact with it. So, please read this article
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