Some of the plasmids used for allelic exchange have colE1/pMB1 ori. Does anybody know which part of the replication cycle is missing in the non permissive strains?
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Alberto Jonathan Martín-Rodríguez
The key is the ori region, which is highly specific for E. coli and its very close relatives. Consequently, in other bacteria different than E. coli (and closest relatives) the plasmid does not have a recognizable origin of replication and thus behaves as a suicide, non-replicable vector.
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Laura Raiger Iustman
Thank you, Alberto!. This I already knew, but do you know which one is the key enzime involved? Why is the ori region so specific? Did E. coli DNA Pol1 recognize an specific sequence? or RNAse H recognize another sequence besides the RNA-DNA hybrid?
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