How can I take subsamples of water from a larger sample in a way that allows me to measure the iron content of the subsample?

I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...

04 February 2021 4,778 1 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

PEI DNA in-vivo transfection concentration?

04 January 2021 6,908 7 View

What would be the recommended color/method for quad-IF?

I'm trying to perform quad-IF staining on frozen tissue sections on a fluorescent widefield (axioplan) microscope. I was wondering what people might suggest for colour/strategy for a co-IF...

13 October 2020 6,863 4 View

How do you remove the tolerance limits when running collinearity statistics in SPSS?

I am using SPSS syntax to determine multicollinearity with predictor variables of interest. When I enter the variables, SPSS is removing some of the variables because the minimum tolerance limit...

02 June 2020 3,639 1 View

How to distinguish different phases on an xrd pattern?

In XRD, how do you distinguish among the crystallized phase, amorphous phase, and a mixture of the two? How will the peak look like in a mixture of a crystalline and amorphous material?

05 May 2020 9,707 7 View

Are there any techniques to embed transmembrane proteins in solid lipid nanoparticles like liposomes or micelles?

25 January 2020 3,991 2 View

Specim FX17 hyperspectral camera/sensor - UAV/drone application?

Hey, I am looking into the feasibility of attaching a Specim FX17 sensor to a multi rotor system. Specs wise it looks possible, sensor is 1.4kg and Matrice M600 max payload is approx 15kg....

16 December 2019 4,169 2 View

Is the calculation of AVE and composite reliability after EFA recommended?

Greetings! The link to the Todd Grande tutorial below demonstrating the calculation of AVE and composite reliability to factor loadings appears to be popular viewing. My question is would you...

08 December 2019 711 4 View

What is the best way to model 9 continuous factors with 2 factors out of the 9 with a non-linear response, i tried using statgraphics, DOE beginner?

Good Day,this is urgent please, i want to use statgraphics trial version to study a part of my work. I am considering different stratified layers in a particular oil reservoir, i considered...

19 October 2019 3,227 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View