Why are microRNA passenger sequences called STAR/ * ?

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Life measurement data of arthropod / insect richness & abundance in Europe?

I was wondering if someone knew a project / institution that gives relatively accurate Live-updates of estimates for the species richness relativ abundance of arthropod / insects species in...

08 September 2017 7,638 1 View

What is a good program to draw mitochondrial genomes?

I am looking for a good and free to use program to draw circular mitochondrial genomes from a file (not genbank). thanks in advance

01 February 2016 4,905 7 View

Which short read aligner do you use?

Hi, I am curious: what method do you use for short read alignment e.g. for microRNA, siRNAs, piRNAs? Or to ask it the other way around: what is the best method for the job in your opinion (and...

31 December 2015 8,787 0 View

Where can I download full genomes of cancer cell lines?

I am looking for full genomes of standard cancer cell-lines for download. I know cosmic but I am unable to bulk-download the data and I believe it is exome data only. Thanks in advance

11 December 2015 3,071 3 View

Has anyone had miRNA qPCR issues because primers were designed based on too short of a miRNA annotation?

If you have any experience given the question - whatever PCR /cDNA approach you used, please comment.

31 December 2014 5,319 13 View

How do you retain DNA, miRNA and mRNA from one sample for NGS?

Hi, So I am interested in a way for generating DNA, smallRNA and mRNA from the same biological sample for NGS (ExomeSeq, smallRNAseq and RNAseq). Essentially I am looking for a protocol/kit that...

10 November 2014 2,034 8 View

What's the optimal sampling for a cancer-normal tissue study?

How many samples (tumor vs. normal tissues) are usually used for NGS based microRNA studies in order to have statistically meaningful results?

08 September 2014 3,516 9 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View