Life measurement data of arthropod / insect richness & abundance in Europe?

I was wondering if someone knew a project / institution that gives relatively accurate Live-updates of estimates for the species richness relativ abundance of arthropod / insects species in...

08 September 2017 7,532 1 View

What is a good program to draw mitochondrial genomes?

I am looking for a good and free to use program to draw circular mitochondrial genomes from a file (not genbank). thanks in advance

01 February 2016 4,790 7 View

Which short read aligner do you use?

31 December 2015 8,666 0 View

Why are microRNA passenger sequences called STAR/ * ?

I am just wondering why and when the passenger strand product of miRNAs has been named "*" / "star" ? Thanks a lot!

06 July 2015 8,071 3 View

Has anyone had miRNA qPCR issues because primers were designed based on too short of a miRNA annotation?

If you have any experience given the question - whatever PCR /cDNA approach you used, please comment.

31 December 2014 5,204 13 View

How do you retain DNA, miRNA and mRNA from one sample for NGS?

Hi, So I am interested in a way for generating DNA, smallRNA and mRNA from the same biological sample for NGS (ExomeSeq, smallRNAseq and RNAseq). Essentially I am looking for a protocol/kit that...

10 November 2014 1,905 8 View

What's the optimal sampling for a cancer-normal tissue study?

How many samples (tumor vs. normal tissues) are usually used for NGS based microRNA studies in order to have statistically meaningful results?

08 September 2014 3,413 9 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View