Bastian Fromm

8 Questions 32 Answers 0 Followers

Questions related from Bastian Fromm

Life measurement data of arthropod / insect richness & abundance in Europe?

I was wondering if someone knew a project / institution that gives relatively accurate Live-updates of estimates for the species richness relativ abundance of arthropod / insects species in...

09 September 2017 7,612 1 View

What is a good program to draw mitochondrial genomes?

I am looking for a good and free to use program to draw circular mitochondrial genomes from a file (not genbank). thanks in advance

02 February 2016 4,875 7 View

Which short read aligner do you use?

Hi, I am curious: what method do you use for short read alignment e.g. for microRNA, siRNAs, piRNAs? Or to ask it the other way around: what is the best method for the job in your opinion (and...

01 January 2016 8,752 0 View

Where can I download full genomes of cancer cell lines?

I am looking for full genomes of standard cancer cell-lines for download. I know cosmic but I am unable to bulk-download the data and I believe it is exome data only. Thanks in advance

12 December 2015 3,045 3 View

Why are microRNA passenger sequences called STAR/ * ?

I am just wondering why and when the passenger strand product of miRNAs has been named "*" / "star" ? Thanks a lot!

07 July 2015 8,161 3 View

Has anyone had miRNA qPCR issues because primers were designed based on too short of a miRNA annotation?

If you have any experience given the question - whatever PCR /cDNA approach you used, please comment.

01 January 2015 5,293 13 View

How do you retain DNA, miRNA and mRNA from one sample for NGS?

Hi, So I am interested in a way for generating DNA, smallRNA and mRNA from the same biological sample for NGS (ExomeSeq, smallRNAseq and RNAseq). Essentially I am looking for a protocol/kit that...

11 November 2014 1,993 8 View

What's the optimal sampling for a cancer-normal tissue study?

How many samples (tumor vs. normal tissues) are usually used for NGS based microRNA studies in order to have statistically meaningful results?

09 September 2014 3,492 9 View