Hi everyone,
I am culturing Jurkat T cells E6.1 obtained from ATCC. This cell line is known to be in suspension forms. The cells were expanded as as suspensions for the first few days after reviving but then became adherent. The culture media used for culturing is made by ATCC protocols (RPMI + 10% FBS). Please let me know if anyone also experienced this and if there are any suggested treatment. Thank you for the help!
Hello Jack Yao
Jurkat cells express integrins alpha5 beta1 and alpha4 beta1 that can mediate cell adhesion. So, they will eventually form some adhesive contact with the substratum although not as strong as in the case of adherent cells. You may refer to the research article below.
Article Beta 1 integrin-dependent binding of Jurkat cells to fibrone...
You may also refer to the article below.
Article Characterization and Applications of Serum-Free Induced Adhe...
Best.
Jurkat cells may loosely adhere to the cell culture flask which can be detached by tapping. If you apparently see completely adherent cells , there is possibility of cross contamination.
One way to avoid adhesion of Jurkat cells is to culture them in flasks in upright position, which I always did. Nevertheless as they divide, they adhere to each other and form floating round aggregates.
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