Why am I getting kind of smiley effect in my SDS-PAGE gel?

04 February 2020 0 8K Report

I was working with a set of proteins and in one of them I got to see a smiley effect in both reducing and non reducing bands. I am using 4-20% biorad precast gel and the concentration of protein is nearly 1mg/ml. Can anyone help me by giving any idea?

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