Arko Bhowal

4 Questions 3 Answers 0 Followers

Questions related from Arko Bhowal

How do you calculate the log fold change of a gene with respect to control in RT-PCR?

I am using 2^(-ΔΔCt) to calculate log fold change of a gene, but some of the values are coming in decimal. For them I am using -1/(the decimal number). Please do let me know what is the rational...

05 July 2021 5,558 5 View

What is the role of cut-off wavelength in fluorescence measurement?

I am using the filter pair of 480/535 and trying to measure fluorescence. The instrument that I am using is Spectramax M2. The Cut-off filter for this pair is showing 530nm in this machine. So...

28 May 2020 893 3 View

Why am I getting kind of smiley effect in my SDS-PAGE gel?

I was working with a set of proteins and in one of them I got to see a smiley effect in both reducing and non reducing bands. I am using 4-20% biorad precast gel and the concentration of protein...

04 February 2020 8,353 0 View

How to quantify protein content in an enzyme having a unit of 1000 U/ml?

I am trying to quantify the protein in terms of mg/ml by BCA assay with an activity of 1000 u/ml.

24 December 2019 4,491 4 View