Kindly check the media preparation once again sir. It should grow in that ; meanwhile,you can also grow in BBM (Bolds basal medium).

I had used the modified Chu-10 medium for Chlorella and I got good biomass of alga. You find in attached file the composition of this media.

Sir, the reduced growth might be due to excess phosphate/ calcium/pH  probably

I think that the pH of the Bg11 is too alcalin for Chlorella, I suggest you should try to test the pH after autoclaving. Otherwise, you can test the Bg11 with bubblking a mixture of Co2 and air (1 to 23% of Co2).

Good luck

R.Jeanjean

Hello,

Could your flasks be too full of media to the point there is not enough CO2 provided to the algae?

Eleonor

May be you have inoculated an old culture (lag phase) which will take more time to show visible growth