How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

Why am I getting 'Infinity' and 'NaN' in my mixed-mode energy release values?

Hi everyone, I am currently running a mixed-mode simulation of a DCB using a user-defined cohesive zone model (UEL), however, the results I am getting don't make sense. I used the same UEL for...

24 February 2021 4,157 1 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

Is there association of SARS-CoV-2 infection rate with meteorological factors?

There are contradictory research papers about the correlation between meteorological parameters and incidence of COVID-19 in some countries worldwide. Is there correlation between the...

15 February 2021 5,806 2 View

What is the best etchant for severely deformed, high purity magnesium?

I have tried using acetic picral and 5% nital solution when observing the microstructure of severely deformed pure magnesium. Unfortunately, I keep getting similar microstructures (as shown below)...

20 January 2021 8,783 4 View

Which material, solution and reagent are needed for HPLC technique?

Of course, there are verity of material, but i need essential material and buffers for this technique. maybe there are some condition for reagent selection that i dont know them. Thank you!

17 January 2021 2,823 13 View

How can I find surface area of a pot which its top and base diameters are 34.5 and 32 cm and its height is 32 cm?

I am measuring the crop water use (ET) by using pots and lettuce crop as plant material, all the data of daily weighted pots are ready but I want to find the surface area of each pot. Can you...

12 January 2021 2,482 4 View

How can I get surface area of a pot which its top diameter is 34.5 cm and 32 cm its base diameter and its height is 32 cm?

I am measuring the crop water use (ET) by using pots and lettuce crop as plant material, all the data of daily weighted pots are ready but I want to find the surface area of each pot. Can please...

12 January 2021 1,726 3 View

Do the freezing reduces the efficacy of the cells for the electroporation ? should i decrease the voltage for the next time?

I'm making reprogramming of MEFs experiment and I used Piggy bac, Sleeping beauty and episomal transposition systems. After electroporation i froze the cells, then i thawed the cells but there...

06 January 2021 5,467 2 View

Do you think that genetic inheritance or the environment is more influential in the development of substance abuse?

Do you think that genetic inheritance or the environment is more influential in the development of substance abuse ?

04 January 2021 1,587 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

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02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View