Prompt research in the direction of "deprivation and adolescents" please?

01 February 2021 6,401 3 View

How does the stress-strain diagram change during continuous dynamic recrystallization ( CDRX) in steel?

It is known that during discontinuous dynamic recrystallization (DDRX) the stress-strain diagram has a stress peak . And how the diagram changes during continuous dynamic recrystallization (CDRX)...

20 March 2019 3,676 4 View

How can I change the y-axis scale of R grofit graphs?

I am trying to plot a Gompertz growth model using grofit. However, ylim in plot() for grofit package is not working. How can I fix it? The code I am using is: library("grofit") data

28 August 2018 3,509 5 View

Recode some items or not?

In my survey, I asked the type of emotions felt by respondants during an event (positive & negative); 4 adjectives/type (Happy, satisfied, excited, dynamic & bored, angry, annoyed, asleep)...

21 July 2018 3,432 2 View

What it could be?

Dear collegues, I would like to know what it could be: at the slide of the leech Hirudo medicilnalis (I tried to find Aeromonas hydrophila Loeffler blue ) I have found a strange microbe. I guess...

29 May 2018 8,009 3 View

No title

25 March 2018 1,375 8 View

What is the best method for the microbe detection in human lyphatic nodes ?

Dear collegues , I need your opinion, because I would like to demonstrate the translocation of microbes out of the gut to the lymphatic nodes (autopcy) of the people died of pneumony associated...

05 March 2018 8,101 4 View

Has anyone done a two plasmid simultaneous transformation in a chemically competent E. coli?

05 August 2017 2,679 6 View

DdPCR for fungal communities

Dear all, has anyone used ddPCR (digital droplet PCR) for assessing fungal communities? Could you recommend any specific primers? Thank you in advance, Respectfully, Tanya

10 July 2017 9,843 1 View

Spectinomycin (LB medium) concentration

30 May 2017 1,499 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View