Hi I am working on bacterial resistance and i want to do cell surface hydrophobicity assay. I tried to do this assay 3-4 time but i am not getting result. The problem is that resistant stains show more absorbance (A) after mixing with hydrocarbon than the initial absorbance(Ao) ie without mixing. But the principle is that resistant strain are more hydrophobic when these strains are mixed with any hydrocarbon the absorbed in hydrocarbon and absorbance reduced from the aqueous phase. but when i fix the initial OD =0.5 and then mix with xylene absorbance increases from 0.5 to 07 or 0.8. Please suggest me what i to do or what is hidden in side this
Dear Sandeep:
Apparently you are using aromatic hydrocarbons? You get the UV spectrum? You do not write the nature of the spectrum. What are hydrocarbons ?. Saturated hydrocarbons do not absorb in the UV. Aromatic hydrocarbons (xylene) extracted aromatic moieties of proteins and therefore increased absorption.
Best regards.
Respected sir
I am using xylene and N Hexadecane. After mixing with them the fixed absorbance 0.5 is increased to 0.6 to 0.8 in different strains. Absorbance was take at 600 nm
That will be done in late stationary phase. Thanks for suggestions...
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