Sandeep Verma

6 Questions 21 Answers 0 Followers

Questions related from Sandeep Verma

Which software can be used for docking of peptide having unusual amino acids?

Hi friends I want to dock a peptide having modified amino acids. I tried with many free software but that are not able to dock that peptide because of modified amino acids. Can any one suggest me...

10 October 2018 7,076 1 View

What can be the loading control for western blotting of bacterial cell lysate.?

Hi i want to do protein expression study of bacteria at different time and sugar. In this condition what can be the loading control but i can not use GAPDH because i am using different sugars....

11 November 2017 307 6 View

Sample preparation for Native PAGE?

Hi please suggest me a procedure of sample preparation for native PAGE. I am working on Gram Positive Bacteria that is highly resistant. I tried to break this with sonication using very high...

12 December 2016 2,852 5 View

SDS-PAGE: Protein bands broaden as it migrates down the gel.?

Hi I am doing bacterial Protein SDS PAGE (Laemmli protocol) using bigger assembly but bands broaden as they migrate down. Solutions compositions Buffer I: 1.5M Tris-HCl pH 8.8 Buffer II: 0.5M...

09 September 2016 6,404 14 View

Gram-positive vector pCASPER availability or what can be the alternative for E. faecalis gene deletion construct through overlap extension PCR ?

Please suggest me that from where i can get Gram-positive, temperature-sensitive mutagenesis vector pCASPER or any alternative that is commercially available.

08 August 2016 7,792 4 View

Why Absorbance is increasing after mixing with hydrocarbon in Hydrophobicity assay (MATH).?

Hi I am working on bacterial resistance and i want to do cell surface hydrophobicity assay. I tried to do this assay 3-4 time but i am not getting result. The problem is that resistant stains show...

05 May 2016 7,333 3 View