Gram-positive vector pCASPER availability or what can be the alternative for E. faecalis gene deletion construct through overlap extension PCR ?

More Sandeep Verma's questions See All

Which software can be used for docking of peptide having unusual amino acids?

Hi friends I want to dock a peptide having modified amino acids. I tried with many free software but that are not able to dock that peptide because of modified amino acids. Can any one suggest me...

09 October 2018 7,100 1 View

What can be the loading control for western blotting of bacterial cell lysate.?

Hi i want to do protein expression study of bacteria at different time and sugar. In this condition what can be the loading control but i can not use GAPDH because i am using different sugars....

10 November 2017 349 6 View

Sample preparation for Native PAGE?

Hi please suggest me a procedure of sample preparation for native PAGE. I am working on Gram Positive Bacteria that is highly resistant. I tried to break this with sonication using very high...

11 December 2016 2,885 5 View

SDS-PAGE: Protein bands broaden as it migrates down the gel.?

Hi I am doing bacterial Protein SDS PAGE (Laemmli protocol) using bigger assembly but bands broaden as they migrate down. Solutions compositions Buffer I: 1.5M Tris-HCl pH 8.8 Buffer II: 0.5M...

08 September 2016 6,434 14 View

Why Absorbance is increasing after mixing with hydrocarbon in Hydrophobicity assay (MATH).?

Hi I am working on bacterial resistance and i want to do cell surface hydrophobicity assay. I tried to do this assay 3-4 time but i am not getting result. The problem is that resistant stains show...

04 May 2016 7,357 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

Jai Ghosh

There is no alternative to pCASPER as it is readily available. Try with Merck biologicals in INDIA.

Sandeep Verma

Sir, i tried to find everywhere but failed. I humbly request you to please guide me

Indeed it is difficult to get. Even Addgene database does not have it. You may try EcoR1 if it works.

Sir, if i am not wrong pCASPER is a vector and EcoR1 is RE.