Whole genome Shotgun sequencing bioinformatics pipelines for all microbial diversity (prokaryotic and eukaryotic) and identify all pathogenic microb?

More Endalkachew Girma Andargie's questions See All

Why NDVI values increased when surface reflectance data was scaled by 0.0000275 and offset value of -0.2?

In my case, it was increased from a range of -0.0966 and +0.541 for unscaled to -1.498 -0.898 for scaled surface reflectance data of combretum-Terminalia woodland

06 May 2023 2,325 0 View

Can I get valuable information from the alkali solution resulted from the alkali treatment of plant fiber using UV/Vis or any other methods?

I treated the fiber with 5% NaHO, @ 80, for an hour, twice. The solution get black in the first and light blue in the second treatment.

26 December 2022 3,321 1 View

During alkali treatment of plant fiber the solution gets blacken in the 1st treatment & light blue in the 2nd . Could some one give me justification?

5 % NaOH (W/V), sample solution proportion of 1:60, @ 80 degree delicious for an hour.

23 December 2022 5,479 3 View

Dear colleagues, How do I extract DNA from rodent fecal sample for whole genome/shotgun sequencing?

Dear colleagues, How do I extract DNA from rodent fecal sample for whole genome/shotgun sequencing? The objective of the research is to characterize the total microbial diversity in the fecal...

19 October 2022 9,136 2 View

Is that good to perform meta regression, sensitivity analysis and funnel plots in meta analysis of prevalence?

I am conducting a systematic review and meta analysis of the prevalence of lost to follow-up among MDR TB patients. I am using STATA 14 for the analysis. I have performed the pooled estimate using...

21 June 2020 3,690 4 View

Is polysystem theory practically feasible for the study of the history of a multi-lingual third world country(Ethiopian) literature?

There are written literatures in Amharic, Oromo, Tigringa and English Languages in Ethiopia and the mainstream in Amharic is so diversified

30 December 2012 9,847 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What role do microorganisms play in nutrient cycling and maintaining soil health in nature and role of microorganisms in the cycling of bio elements?

31 July 2024 3,965 0 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

How do pollutants in soil affect soil microorganisms and role do microorganisms play in nutrient cycling and soil fertility?

31 July 2024 368 2 View

What's biological importance of bacteria &role of microbes improve crop productivity &soil health &role of bacteria as decomposers incycling nutrient?

What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?

31 July 2024 3,479 5 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?

31 July 2024 8,483 8 View

Abhijeet Singh

These articles can agive you a good initial idea

https://doi.org/10.1128/msystems.00432-22

Article Development and Optimization of an Unbiased, Metagenomics-Ba...

Article Combination of Whole Genome Sequencing and Metagenomics for ...

Endalkachew Girma Andargie

Dear Dr Abhijeet Singh, thank you so much.