Who is the volume of enzyme estimated?

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Engelbert Buxbaum

The bottle of your enzyme should indicate what the stock concentration is. Then desired volume * desired concentration / stock concentration is your answer.

Abdul Aziz Khalfan Al Bahri

sorry for the typo mistake, I mean 'how' not 'who'

how is the volume of enzyme estimated?

Michael J. Benedik

It will entirely depend upon the amount of substrate to be hydrolyzed in the sample assuming reaction conditions and time are fairly standardized and reasonably optimal.