10 October 2016 2 7K Report

More Leonhard Voltmer's questions See All
Procedure of Ammonium sulfate precipitation of ACE2 in Calu-3 cells?

Hello, I am trying to find a protocol that looks at the extraction and purification of ACE2 inside the cell. We have the protocol for the affinity chromatography, but we are having trouble...

23 May 2024 4,342 1 View

How to estimate the bond distances in metal complexes using covalent radii?

Hello, I need to estimate the bond distances of atoms in metal complexes by using the covalent and/or van-der-Waals radii. Currently my wild guess after some thoughts is to weigh the sum of the...

13 August 2020 7,496 3 View

Which western blot antibodies to screen mechanisms in CRC cell lines?

I want to make a list of 150 western blot ABs to screen for interesting mechanisms/pathways in CRC cell lines (HT29 and HCT116 which we will treat with some drugs). Which pathways should be...

17 March 2018 3,574 2 View

What virus concentration should i use for the lentiviral transduction of human primary T cells?

Dear friends, I would like to do a lentiviral transduction (VsVg, Delta 89) of human primary T cells. Do you have any experience with that? What concentration would you use? And do you have any...

27 November 2016 1,979 3 View

Control unstable zeros with unstable poles

I am having trouble designing a controller to stabilize a non-minimum phase system. I am using an IIR filter composed of >10 biquads, trying to compensate for the shape of my actuator's...

21 July 2016 9,586 3 View

Short-term storage of E. coli: +4, -20, -80°C?

Hi everyone, for a set of experiments I would like to store E. coli for four to five days. I did some literature recherche on short term storage of bacteria found a lot of different ways to do...

01 June 2016 7,831 9 View

How to reduce unspecific binding in an intracellular click-reaction?

Good morning, we are performing an intracellular click-reaction using an azide coupeled to an intracellular protein and a TAMRA alkyne (after cultivation, the cells are fixed, permeabilized,...

22 June 2015 4,767 6 View

Is polycarbonate attacked by chloroform?

Hi everyone, I want to extract lipids via the Folch-method. For this, I have to incubate cells with a chloroform-methanol-mixture. After this, I need to centrifuge the suspension. We have a nice...

08 April 2015 8,996 5 View

What group therapy treatments/techniques demonstrate the greatest efficacy with batterers?

What group therapy (for batterers, mandated or self-selected) techniques and or interventions are widely used due to positive evidence based performance outcomes?

03 November 2014 6,642 8 View

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Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

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Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

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The Origin of Human Language?

I attended a lecture at the Baylor College of Medicine (~ 2019) where one of the questions was “Does birdsong have anything to do with human language?” Noam Chomsky would say, “Absolutely not!”...

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Posthoc test lettering in JAMOVI?

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Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

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What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

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Can we eliminate the stress singularity at the tip of the crack by manipulating the elastic constants?

The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.

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How do I get people to interview on their motivations for writing graffiti in washrooms in a university?

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What effects of Autonomous Language Learning can be shown regarding linguistic competence and communicative skills?

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