Who could help me with a protocol to optimize bigdye terminator kit usage? My fragments are no longer than 300bp.

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Musharraf Jelani

Dear Nelson see the page attached Bigdye Terminator Protocol. I have used and optimized this protocol during my PhD studies. This page is from my PhD thesis. Best of luck

Vincenzo Landi

look here..

http://www.woodrow.org/teachers/BIOLOGY03/Projects/group1/word/BDCycleSeqprotocols.pdf

Nelson Carvalho Filho

Thank you very much for indeed for the tips. In fact .5 ul is the limit.