I need some strains of Agrobaterium rhizogenes
I'm trying Multifn software to analyse some molecular properties. For doing, I need .wfn or .fch or .molden input format file but I only have .cif and .pdb files. Unfortunately, I don't have...
03 March 2021 2,557 10 View
Hi all, I have been facing problems with colony pcr for agrobacterium colonies. I did site directed mutagenesis, cloned to pcambia, sequenced and confirmed and then transformed to Agrobacterium....
02 June 2024 6,517 5 View
Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...
26 May 2024 9,304 3 View
I am confused regarding the desired edit efficiency of PEP2 for transversion on rice plants using Agrobacterium.
21 May 2024 4,332 0 View
Strain: LBA4404 Growing media: YEB (pH 7) Temperature: 28°C, 200 rpm (overnight culture) Antibiotic use: Kanamycin and Streptomycin
13 May 2024 7,726 2 View
I could've sworn that I read a protocol where strained plant "juices" were added to agrobacterium cultures in lieu of pure acetosyringone. If you have heard of anything like this, please let me...
06 May 2024 9,001 0 View
Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...
26 April 2024 8,440 3 View
Hello. I am trying to culture Agrobacterium GV 3103 and EHA105 in 15 mL LB media with the antibiotics kanamycin and rifampicin (100 ug/mL & 20 ug/mL)(added 200 uL of previously normally...
25 January 2024 812 5 View
We tried to do activation-tagging in Arabidopsis using the plasmid pSKi015. Nevertheless, we failed to obtain positive clones in GV3101? The recommended Agrobacterium strain for pSKI015 is GV3101...
21 January 2024 1,246 2 View
We have numerous previous articles and specific protocols by which you can regenerate transgenic rice plants. However, following the existing methods/protocol, if you do not get the positive...
01 November 2023 9,301 3 View
I have AGL1 agrobacterium, it's not competent. i need to do it competent. any recommended protocols. i tried the TSS we use for E.coli but not working. also i tried the cacl2 protocol and don't...
29 October 2023 7,321 5 View