We have numerous previous articles and specific protocols by which you can regenerate transgenic rice plants. However, following the existing methods/protocol, if you do not get the positive outcome, I.e., no transgenic plants, what would be the possible reasons behind the failure to produce transgenic ones?
Roksana Aftab Three questions:
1) Have you got any other transgenic plants besides rice with the same Ag strains/plasmids using the same or other protocols?
2) Have you got high frequency rice regeneration with the same genotypes without transformation?
3) Have you done a transient expression with the same Ag strains/plasmids?
Dear Alex Ignatov
1) yes. On the same genres of plants, this volume worked.
2) The frequency is not high but considerable.
3) yes.
Thank you.
Dear Roksana
Rice genetic transformation varies from one cultivar to another, for some cultivars transformation efficiency (japonica Nipponbare) is better while others have low or very little (Indica rice). Transformation can be improved by taking immature embryos as explant.
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