We tried to do activation-tagging in Arabidopsis using the plasmid pSKi015. Nevertheless, we failed to obtain positive clones in GV3101? The recommended Agrobacterium strain for pSKI015 is GV3101 pMP90RK. Does GV3101 also work? Can carbenicillin be used for screen the positive clone instead of Ampicillin?Thanks!

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